1 time per night, after cleansing, use bristles to extract the eyelash growth liquid, smear from the eyelash root ministry to the top. Use twice a day, AM and PM, more visible results.General users, the shortest 7 days, can feel the eyelash growth, become thick, black. Use 16 weeks can fully feel the effect, In order to maintain the effect, need sustainable use. If you don't use continuously, a few weeks or a few months (hair growth cycle), eyelash will revert back to its original state.